An antimicrobial thiopeptide producing novel actinomycetes streptomyces terrae sp. nov., isolated from subsurface soil of arable land

Abstract An antimicrobial producing Gram-positive, aerobic, non-motile and filamentous actinobacterial strain SKN60T was isolated from soil The isolate exhibited 99.3 99.0% identity with Streptomyces laurentii ATCC 31255T S. roseicoloratus TRM 44457T, respectively, in 16S rRNA gene sequence analysis. However, the genome displayed maximum ANI (88.45%) AAI (85.61%) 44457T. Similarly, dDDH showed 33.7% It formed a cluster 44457T phylogenomic tree. Cell wall analysis revealed presence of diphosphatidylglycerol, phosphatidylethanolamine phosphatidylcholine as major polar lipids diaminopimelic acid diagnostic diamino acid. Major fatty acids were iso-C15:0, anteiso-C15:0, iso-C16:0. G+C content found to be 72.3 mol%. Genome using antiSMASH database occurrence thiopeptide biosynthesis 94% similarity berninamycin bernensis UC5144. mass 1146 Da is identical berninamycin. But subtle differences observed leader peptide Notably, not validly reported thus only containing BGC no other phylogenetic relative had it. Additionally, differed phenotypic genetic characteristics all relatives genus Streptomyces. Therefore, it proposed novel species name terrae sp. nov. (=MTCC 13163T; = JCM 35768T).